RT Journal Article
SR Electronic
T1 Centriole triplet microtubules are required for stable centriole formation and inheritance in human cells
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 147975
DO 10.1101/147975
A1 Jennifer T. Wang
A1 Dong Kong
A1 Christian R. Hoerner
A1 Jadranka Loncarek
A1 Tim Stearns
YR 2017
UL http://biorxiv.org/content/early/2017/06/08/147975.abstract
AB Centrioles are composed of long-lived microtubules arranged in nine triplets. In unicellular eukaryotes, loss of the noncanonical tubulins, delta-tubulin and epsilon tubulin, result in loss of the triplet microtubule structure. However, the contribution of triplet microtubules to mammalian centriole formation and stability is unknown. Here, we report the first characterization of delta-tubulin and epsilon-tubulin null human cells. Centrioles in cells lacking either delta-tubulin or epsilon-tubulin lack triplet microtubules and fail to undergo centriole maturation. These aberrant centrioles are formed de novo each cell cycle, but are unstable and do not persist to the next cell cycle, leading to a futile cycle of centriole formation and disintegration. Disintegration can be suppressed by paclitaxel treatment. Delta-tubulin and epsilon-tubulin physically interact, indicating that these tubulins act together to maintain triplet microtubules and that these are necessary for inheritance of centrioles from one cell cycle to the next.