PT  - JOURNAL ARTICLE
AU  - Monica Pia Caggiano
AU  - Xiulian Yu
AU  - Neha Bhatia
AU  - André Larsson
AU  - Hasthi Ram
AU  - Carolyn Ohno
AU  - Pia Sappl
AU  - Elliot M. Meyerowitz
AU  - Henrik Jönsson
AU  - Marcus G. Heisler
TI  - Cell type boundaries organize plant development
AID  - 10.1101/126466
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 126466
4099  - http://biorxiv.org/content/early/2017/06/12/126466.short
4100  - http://biorxiv.org/content/early/2017/06/12/126466.full
AB  - In plants the dorsoventral boundary of leaves defines an axis of symmetry through the centre of the organ separating the top (dorsal) and bottom (ventral) tissues. Although the positioning of this boundary is critical for leaf morphogenesis, how the boundary is established and how it influences development remains unclear. Using live-imaging and perturbation experiments we show that leaf orientation, morphology and position are pre-patterned by HD-ZIPIII and KAN gene expression in the shoot, leading to a model in which dorsoventral genes coordinate to regulate plant development by localizing auxin response between their expression domains. However we also find that auxin levels feedback on dorsoventral patterning by spatially organizing HD-ZIPIII and KAN expression in the shoot periphery. By demonstrating that the regulation of these genes by auxin also governs their response to wounds, our results also provide a parsimonious explanation for the influence of wounds on leaf dorsoventrality.