PT  - JOURNAL ARTICLE
AU  - Zheng, Zhihao
AU  - Lauritzen, J. Scott
AU  - Perlman, Eric
AU  - Robinson, Camenzind G.
AU  - Nichols, Matthew
AU  - Milkie, Daniel
AU  - Torrens, Omar
AU  - Price, John
AU  - Fisher, Corey B.
AU  - Sharifi, Nadiya
AU  - Calle-Schuler, Steven A.
AU  - Kmecova, Lucia
AU  - Ali, Iqbal J.
AU  - Karsh, Bill
AU  - Trautman, Eric T.
AU  - Bogovic, John
AU  - Hanslovsky, Philipp
AU  - Jefferis, Gregory S. X. E.
AU  - Kazhdan, Michael
AU  - Khairy, Khaled
AU  - Saalfeld, Stephan
AU  - Fetter, Richard D.
AU  - Bock, Davi D.
TI  - A complete electron microscopy volume of the brain of adult <em>Drosophila melanogaster</em>
AID  - 10.1101/140905
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 140905
4099  - http://biorxiv.org/content/early/2017/06/13/140905.short
4100  - http://biorxiv.org/content/early/2017/06/13/140905.full
AB  - Drosophila melanogaster has a rich repertoire of innate and learned behaviors. Its 100,000–neuron brain is a large but tractable target for comprehensive neural circuit mapping. Only electron microscopy (EM) enables complete, unbiased mapping of synaptic connectivity; however, the fly brain is too large for conventional EM. We developed a custom high-throughput EM platform and imaged the entire brain of an adult female fly. We validated the dataset by tracing brain-spanning circuitry involving the mushroom body (MB), intensively studied for its role in learning. Here we describe the complete set of olfactory inputs to the MB; find a new cell type providing driving input to Kenyon cells (the intrinsic MB neurons); identify neurons postsynaptic to Kenyon cell dendrites; and find that axonal arbors providing input to the MB calyx are more tightly clustered than previously indicated by light-level data. This freely available EM dataset will significantly accelerate Drosophila neuroscience.- A complete adult fruit fly brain was imaged, using electron microscopy (EM)- The EM volume enables brain-spanning mapping of neuronal circuits at the synaptic level- Olfactory projection neurons cluster more tightly in mushroom body calyx than expected from light-level data- The primary postsynaptic targets of Kenyon cells (KCs) in the MB are other KCs, as well as the anterior paired lateral (APL) neuron- A newly discovered cell type, MB-CP2, integrates input from several sensory modalities and provides microglomerular input to KCs in MB calyx- A software pipeline was created in which EM-traced skeletons can be searched for within existing large-scale light microscopy (LM) databases of neuronal morphology, facilitating cell type identification and discovery of relevant genetic driver lines