PT  - JOURNAL ARTICLE
AU  - Dvir Aran
AU  - Zicheng Hu
AU  - Atul J. Butte
TI  - xCell: Digitally portraying the tissue cellular heterogeneity landscape
AID  - 10.1101/114165
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 114165
4099  - http://biorxiv.org/content/early/2017/06/15/114165.short
4100  - http://biorxiv.org/content/early/2017/06/15/114165.full
AB  - Tissues are complex milieu consisting of numerous cell-types. Numerous recent methods attempt to enumerate cell subsets from transcriptomes. However, available method used limited source for training and displayed only partial portrayal of the full cellular landscape. Here we present xCell, a novel gene-signature based method for inferring 64 immune and stroma cell-types. We harmonized 1,822 pure human cell-types transcriptomes from various sources, employed curve fitting approach for linear comparison of cell-types, and introduced a novel spillover compensation technique for separating between cell-types. Using extensive in silico analyses and comparison to cytometry immunophenotyping we show that xCell outperforms other methods: http://xCell.ucsf.edu/.