PT  - JOURNAL ARTICLE
AU  - Swati Parekh
AU  - Christoph Ziegenhain
AU  - Beate Vieth
AU  - Wolfgang Enard
AU  - Ines Hellmann
TI  - zUMIs
AID  - 10.1101/153940
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 153940
4099  - http://biorxiv.org/content/early/2017/06/22/153940.short
4100  - http://biorxiv.org/content/early/2017/06/22/153940.full
AB  - RNA sequencing is increasingly performed with less starting material and at a higher sample throughput, e.g. to analyse single-cell transcriptomes. In this context, unique molecular identifiers (UMIs) are used to reduce amplification noise and sample-specific barcodes are used to track libraries. Here, we present a fast and flexible pipeline to process data from such RNA-seq protocols.Availability: https://github.com/sdparekh/zUMIs