TY - JOUR T1 - A practical guide to CRISPR/Cas9 genome editing in Lepidoptera JF - bioRxiv DO - 10.1101/130344 SP - 130344 AU - Linlin Zhang AU - Robert D. Reed Y1 - 2017/01/01 UR - http://biorxiv.org/content/early/2017/06/22/130344.abstract N2 - CRISPR/Cas9 genome editing has revolutionized functional genetic work in many organisms and is having an especially strong impact in emerging model systems. Here we summarize recent advances in applying CRISPR/Cas9 methods in Lepidoptera, with a focus on providing practical advice on the entire process of genome editing from experimental design through to genotyping. We also describe successful targeted GFP knockins that we have achieved in butterflies. Finally, we provide a complete, detailed protocol for producing targeted long deletions in butterflies. ER -