RT Journal Article
SR Electronic
T1 Bottled aqua incognita: Microbiota assembly and dissolved organic matter diversity in natural mineral waters
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 154732
DO 10.1101/154732
A1 Celine C. Lesaulnier
A1 Craig W. Herbold
A1 Claus Pelikan
A1 David Berry
A1 Cédric Gérard
A1 Xavier Le Coz
A1 Sophie Gagnot
A1 Jutta Niggemann
A1 Thorsten Dittmar
A1 Gabriel A. Singer
A1 Alexander Loy
YR 2017
UL http://biorxiv.org/content/early/2017/06/23/154732.abstract
AB Background Non-carbonated natural mineral waters contain microorganisms that regularly grow after bottling despite low concentrations of dissolved organic matter (DOM). Yet, the compositions of bottled water microbiota and organic substrates that fuel microbial activity, and how both change after bottling, are still largely unknown.Results We performed a multifaceted analysis of microbiota and DOM diversity in twelve natural mineral waters from six European countries. 16S rRNA gene-based analyses showed that less than ten species-level operational taxonomic units (OTUs) dominated the bacterial communities in the water phase and associated with the bottle wall after a short phase of post-bottling growth. Members of the betaproteobacterial genera Curvibacter, Aquabacterium, and Polaromonas (Comamonadaceae) grew in most waters and represent ubiquitous, mesophilic, heterotrophic aerobes in bottled waters. Ultrahigh-resolution mass spectrometry of DOM in bottled waters and their corresponding source waters identified thousands of molecular formulae characteristic of mostly refractory, soil-derived DOM.Conclusions The bottle environment, including source water physicochemistry, selected for growth of a similar low-diversity microbiota across various bottled waters. Relative abundance changes of hundreds of multi-carbon molecules were related to growth of less than ten abundant OTUs. We thus speculate that individual bacteria cope with oligotrophic conditions by simultaneously consuming diverse DOM molecules.