RT Journal Article SR Electronic T1 Mapping Loci that Control Tuber and Foliar Symptoms Caused by PVY in Autotetraploid Potato (Solanum tuberosum L.) JF bioRxiv FD Cold Spring Harbor Laboratory SP 156539 DO 10.1101/156539 A1 Washington da Silva A1 Jason Ingram A1 Christine A. Hackett A1 Joseph J. Coombs A1 David Douches A1 Glenn Bryan A1 Walter De Jong A1 Stewart Gray YR 2017 UL http://biorxiv.org/content/early/2017/06/27/156539.abstract AB Potato tuber necrotic ringspot disease (PTNRD) is a tuber deformity associated with infection by the tuber necrotic strain of Potato virus Y (PVYNTN). PTNRD negatively impacts tuber quality and marketability and poses a serious threat to seed and commercial potato production worldwide. PVYNTN symptoms differ in the cultivars Waneta and Pike: Waneta expresses severe PTNRD and foliar mosaic with vein and leaf necrosis, whereas Pike does not express PTNRD and mosaic is the only foliar symptom. To map loci that influence tuber and foliar symptoms, 236 F1 progeny of a cross between Waneta and Pike were inoculated with PVYNTN isolate NY090029 and genotyped using 12,808 Potato SNPs. Foliar symptom type and severity were monitored for 10 weeks, while tubers were evaluated for PTNRD expression at harvest and again after 60 days in storage. Pairwise correlation analyses indicate a strong association between PTNRD and vein necrosis (τ = 0.4195). QTL analyses revealed major-effect QTLs on chromosomes 4 and 5 for mosaic, 4 for PTNRD, and 5 for foliar-necrosis symptoms. Locating QTLs associated with PVY-related symptoms provides a foundation for breeders to develop markers that can be used to screen out potato clones with undesirable phenotypes, e.g., those likely to develop PTNRD or to be symptomless carriers of PVY.