RT Journal Article SR Electronic T1 Analysis of behaviour in the Active Allothetic Place Avoidance task based on cluster analysis of the rat movement motifs JF bioRxiv FD Cold Spring Harbor Laboratory SP 157859 DO 10.1101/157859 A1 Tiago V. Gehring A1 Malgorzata J. Wesierska A1 Daniel K. Wójcik A1 Eleni Vasilaki YR 2017 UL http://biorxiv.org/content/early/2017/06/30/157859.abstract AB The Active Allothetic Place Avoidance test (AAPA) is a useful tool to study spatial memory in a dynamic world. In this task a rat, freely moving on a rotating circular arena, has to avoid a sector where shocks are presented. The standard analysis of memory performance in the AAPA task relies on evaluating individual performance measures. Here we present a new method of analysis for the AAPA test that focuses on the movement paths of the animals and utilizes a clustering algorithm to automatically extract the stereotypical types of behaviour as reflected in the recorded paths. We apply the method to experiments that study the effect of silver nanoparticles (AgNPs) on the reference memory and identify six major classes of movement motifs not previously described in AAPA tests. The method allows us to analyse the data with no prior expectations about the motion to be seen in the experiments.