PT  - JOURNAL ARTICLE
AU  - Claudia Fredolini
AU  - Sanna Byström
AU  - Laura Sanchez-Rivera
AU  - Marina Ioannou
AU  - Davide Tamburro
AU  - Rui M. Branca
AU  - Janne Lehtiö
AU  - Peter Nilsson
AU  - Jochen M. Schwenk
TI  - Mass spectrometry based qualification of antibodies for plasma proteomics
AID  - 10.1101/158022
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 158022
4099  - http://biorxiv.org/content/early/2017/06/30/158022.short
4100  - http://biorxiv.org/content/early/2017/06/30/158022.full
AB  - There is a strong need for procedures that enable context and application dependent validation of antibodies. Here we describe a high-throughput approach for the detailed assessment of the selectivity of antibodies in plasma by PLasma Immunocapture Mass Spectrometry (PLIMS). The utility of PLIMS is demonstrated by determining the enrichment profiles of 157 antibodies targeting 120 proteins in EDTA plasma. Applying four classification categories (ON-target, CO-target, OFF-target and NO-target), it was found that 60% (44/60) of antibodies directed against denoted plasma proteins qualified for plasma assays. Among these, 85% (60/71) co-enriched another protein besides their respective target. As shown for several antibodies against IGFBP2, PLIMS was furthermore capable to describe known and explore novel protein complexes in plasma. In summary, PLIMS provides detailed insights into antibody selectivity in the context of plasma, thus will contribute as a valuable procedure towards to the generation of more reliable affinity-based plasma proteomics data.