RT Journal Article
SR Electronic
T1 MassBlast: A workflow to accelerate RNA-seq and DNA database analysis
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 131953
DO 10.1101/131953
A1 André Veríssimo
A1 Jean-Etienne Bassard
A1 Alice Julien-Laferrière
A1 Marie-France Sagot
A1 Susana Vinga
YR 2017
UL http://biorxiv.org/content/early/2017/07/03/131953.abstract
AB Summary Current workflows for sequence analysis heavily depend on user input and manual curation. New specialized tools and methods are appearing all the time, but the actions required for a full analysis are disconnected and very time-consuming. The software we propose, MassBlast, combines BLAST+ and an automated workflow analysis to filter the results and significantly improve the annotation of multiple sequencing databases for exploring new biosynthetic pathways and new protein families, among other applications. MassBlast is fully configurable and reproducible.Availability and Implementation The MassBlast package is written in Ruby. Source code and releases are freely available from Github (https://github.com/averissimo/mass-blast) for all major platforms (Linux, MS Windows and OS X) under the GPLv3 license.Contact andre.verissimo{at}tecnico.ulisboa.pt