PT - JOURNAL ARTICLE AU - Congwei Wang AU - Fabian Schmich AU - Julie Weidner AU - Niko Beerenwinkel AU - Anne Spang TI - Context-dependent deposition and regulation of mRNAs in P-bodies AID - 10.1101/159376 DP - 2017 Jan 01 TA - bioRxiv PG - 159376 4099 - http://biorxiv.org/content/early/2017/07/04/159376.short 4100 - http://biorxiv.org/content/early/2017/07/04/159376.full AB - Cells respond to stress by remodeling their transcriptome through transcription and degradation. Xrn1p-dependent degradation in P-bodies is the most prevalent pathway. Yet, P-bodies may facilitate not only decay but also act as storage compartment. However, which and how mRNAs are selected into different degradation pathways and what determines the fate of any given mRNA in P-bodies remain largely unknown. We devised a new method to identify both common and stress-specific mRNA subsets associated with P-bodies. mRNAs targeted for degradation to P-bodies, decayed with different kinetics. Moreover, the localization of a specific set of mRNAs to P-bodies under glucose deprivation was obligatory to prevent decay. Depending on its client mRNA, the RNA binding protein Puf5p either promoted or inhibited decay. The Puf5p-dependent storage of a subset of mRNAs in P-bodies under glucose starvation may be beneficial with respect to chronological lifespan.