RT Journal Article SR Electronic T1 Context-dependent deposition and regulation of mRNAs in P-bodies JF bioRxiv FD Cold Spring Harbor Laboratory SP 159376 DO 10.1101/159376 A1 Congwei Wang A1 Fabian Schmich A1 Julie Weidner A1 Niko Beerenwinkel A1 Anne Spang YR 2017 UL http://biorxiv.org/content/early/2017/07/04/159376.abstract AB Cells respond to stress by remodeling their transcriptome through transcription and degradation. Xrn1p-dependent degradation in P-bodies is the most prevalent pathway. Yet, P-bodies may facilitate not only decay but also act as storage compartment. However, which and how mRNAs are selected into different degradation pathways and what determines the fate of any given mRNA in P-bodies remain largely unknown. We devised a new method to identify both common and stress-specific mRNA subsets associated with P-bodies. mRNAs targeted for degradation to P-bodies, decayed with different kinetics. Moreover, the localization of a specific set of mRNAs to P-bodies under glucose deprivation was obligatory to prevent decay. Depending on its client mRNA, the RNA binding protein Puf5p either promoted or inhibited decay. The Puf5p-dependent storage of a subset of mRNAs in P-bodies under glucose starvation may be beneficial with respect to chronological lifespan.