RT Journal Article
SR Electronic
T1 Zinc storage granules in the Malpighian tubules of <em>Drosophila melanogaster</em>
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 159558
DO 10.1101/159558
A1 Carlos Tejeda-Guzmán
A1 Abraham Rosas-Arellano
A1 Thomas Kroll
A1 Samuel M. Webb
A1 Martha Barajas-Aceves
A1 Beatriz Osorio
A1 Fanis Missirlis
YR 2017
UL http://biorxiv.org/content/early/2017/07/04/159558.abstract
AB Membrane transporters and sequestration mechanisms concentrate metal ions differentially into discrete subcellular microenvironments for usage in protein cofactors, signaling, storage, or excretion. Here we identify zinc storage granules as the insect’s major zinc reservoir in primary Malpighian tubule epithelial cells of Drosophila melanogaster. The concerted action of Adaptor Protein-3, Rab32, HOPS and BLOC complexes as well as of the white-scarlet (ABCG2-like) and ZnT35C transporters is required for zinc storage granule biogenesis. Due to similar lysosome related organelle defects, patients with Hermansky-Pudlak syndrome may lack zinc granules in beta pancreatic cells, intestinal paneth cells and presynaptic vesicles of hippocampal mossy fibers.