RT Journal Article
SR Electronic
T1 CRISPR/Cas9-APEX-mediated proximity labeling enables discovery of proteins associated with a predefined genomic locus in living cells
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 159517
DO 10.1101/159517
A1 Samuel A Myers
A1 Jason Wright
A1 Feng Zhang
A1 Steven A Carr
YR 2017
UL http://biorxiv.org/content/early/2017/07/04/159517.abstract
AB The activation or repression of a gene’s expression is primarily controlled by changes in the proteins that occupy its regulatory elements. The most common method to identify proteins associated with genomic loci is chromatin immunoprecipitation (ChIP). While having greatly advanced our understanding of gene expression regulation, ChIP requires specific, high quality, IP-competent antibodies against nominated proteins, which can limit its utility and scope for discovery. Thus, a method able to discover and identify proteins associated with a particular genomic locus within the native cellular context would be extremely valuable. Here, we present a novel technology combining recent advances in chemical biology, genome targeting, and quantitative mass spectrometry to develop genomic locus proteomics, a method able to identify proteins which occupy a specific genomic locus.