TY - JOUR T1 - The Detection of Phase Amplitude Coupling During Sensory Processing JF - bioRxiv DO - 10.1101/163006 SP - 163006 AU - R.A Seymour AU - G. Rippon AU - K. Kessler Y1 - 2017/01/01 UR - http://biorxiv.org/content/early/2017/07/13/163006.abstract N2 - There is increasing interest in understanding how the phase and amplitude of distinct neural oscillations might interact to support dynamic communication within the brain. In particular, previous work has demonstrated a coupling between the phase of low frequency oscillations and the amplitude (or power) of high frequency oscillations during certain tasks, termed phase amplitude coupling (PAC). For instance, during visual processing in humans, PAC has been reliably observed between ongoing alpha (8-13Hz) and gamma-band (>40Hz) activity. However, the application of PAC metrics to electrophysiological data can be challenging due to numerous methodological issues and lack of coherent approaches within the field. Therefore, in this article we outline the various analysis steps involved in detecting PAC, using an openly available MEG dataset from 16 participants performing an interactive visual task. Firstly, we localised gamma and alpha-band power using the Fieldtrip toolbox, and extracted time courses from area V1, defined using a multimodal parcellation scheme. These V1 responses were analysed for changes in alpha-gamma PAC, using four common algorithms. Results showed an increase in gamma (40-100Hz) - alpha (7-13Hz) PAC in response to the visual grating stimulus, though specific patterns of coupling were somewhat dependent upon the algorithm employed. Additionally, post-hoc analyses showed that these results were not driven by the presence of non-sinusoidal oscillations, and that trial length was sufficient to obtain reliable PAC estimates. Finally, throughout the article, methodological issues and practical guidelines for ongoing PAC research will be discussed. ER -