RT Journal Article SR Electronic T1 The diversity, structure and function of heritable adaptive immunity sequences in the Aedes aegypti genome JF bioRxiv FD Cold Spring Harbor Laboratory SP 127498 DO 10.1101/127498 A1 Whitfield, Zachary J. A1 Dolan, Patrick T. A1 Kunitomi, Mark A1 Tassetto, Michel A1 Seetin, Matthew G. A1 Oh, Steve A1 Heiner, Cheryl A1 Paxinos, Ellen A1 Andino, Raul YR 2017 UL http://biorxiv.org/content/early/2017/07/15/127498.abstract AB The Aedes aegypti mosquito is a major vector for arboviruses including dengue, chikungunya and Zika virus. Combating the spread of these viruses requires a more complete understanding of the mosquito immune system. Recent studies have implicated genomic endogenous viral elements (EVEs) derived from non-retroviral RNA viruses in insect immunity. Because these elements are inserted into repetitive regions of the mosquito genome, their large-scale structure and organization with respect to other genomic elements has been difficult to resolve with short-read sequencing. To better define the origin, diversity and biological role of EVEs, we employed single-molecule, real-time sequencing technology to generate a high quality, long-read assembly of the Ae. aegypti-derived Aag2 cell line genome. We leverage the quality and contiguity of this assembly to characterize the diversity and genomic context of EVEs in the genome of this important model system. We find that EVEs in the Aag2 genome are acquired through recombination by LTR retrotransposons, and organize into larger loci (>50kbp) characterized by high LTR density. These EVE containing loci are associated with increased transcription factor binding sight density and increased production of anti-genomic piRNAs. We also detected piRNA processing corresponding to on-going viral infection. This global view of EVEs and piRNA responses demonstrates the ubiquity and diversity of these heritable elements that define small-RNA mediated antiviral immunity in mosquitoes.