PT  - JOURNAL ARTICLE
AU  - Gupta, Vimlesh
AU  - Saxena, Hari Mohan
TI  - Isolation and characterization of BpL1, a broad acting lytic bacteriophage against Brucella
AID  - 10.1101/165076
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 165076
4099  - http://biorxiv.org/content/early/2017/07/18/165076.short
4100  - http://biorxiv.org/content/early/2017/07/18/165076.full
AB  - We have isolated a new broad acting lytic brucellaphage (BpL1) from the sewage of a dairy farm. The phage lysed all the 12 Brucella abortus field isolates, B. abortus strain 99 and Brucella melitensis but did not lyse any of the heterologous species tested viz. Staphylococcus aureus, Pasteurella multocida, Escherichia coli, and Salmonella species. Streaking the plaques on Brucella lawn gave clear zones along the streak lines. The plaques were circular with a diameter of 0.5- 3.0 mm. At a concentration of 10−4 the phage count was 4.5 × 106 plaques per ml. It was a tailed phage with icosahedral head (62.2 nm in diameter and 73.71 nm in length), and the head to tail length was 229.21 nm. The phage belonged to the order Caudovirales and family Siphoviridae. It was inactivated within one hour at 55°C and within 4 hours at −20°C. Treatment at pH 2 for 4 hours and at pH 4 for 12 hours inactivated it. It was inactivated after 4 hours exposure to sunlight, and within 4 minutes by UV light. Chloroform and Sodium Dodecyl Sulfate inactivated it within 15 minutes. Lysozyme inactivated it within 1 hour whereas RNase treatment did not affect its activity.