PT  - JOURNAL ARTICLE
AU  - Spyros Darmanis
AU  - Steven A. Sloan
AU  - Derek Croote
AU  - Marco Mignardi
AU  - Sophia Chernikova
AU  - Peyman Samghababi
AU  - Ye Zhang
AU  - Norma Neff
AU  - Mark Kowarsky
AU  - Christine Caneda
AU  - Gordon Li
AU  - Steven Chang
AU  - Ian David Connolly
AU  - Yingmei Li
AU  - Ben Barres
AU  - Melanie Hayden Gephart
AU  - Stephen R. Quake
TI  - Single-Cell RNAseq analysis of infiltrating neoplastic cells at the migrating front of human glioblastoma
AID  - 10.1101/165811
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 165811
4099  - http://biorxiv.org/content/early/2017/07/19/165811.short
4100  - http://biorxiv.org/content/early/2017/07/19/165811.full
AB  - Glioblastoma is the most common primary brain cancer in adults and is notoriously difficult to treat due to its diffuse nature. We performed single-cell RNAseq on 3589 cells in a cohort of four patients. We obtained cells from the tumor core as well as surrounding peripheral tissue. Our analysis revealed cellular variation in the tumor’s genome and transcriptome, We were able to identify infiltrating neoplastic cells in regions peripheral to the core lesions. Despite the existence of significant heterogeneity among neoplastic cells, we found that infiltrating GBM cells share a consistent gene signature between patients, suggesting a common mechanism of infiltration. Additionally, in investigating the immunological response to the tumors, we found transcriptionally distinct myeloid cell populations residing in the tumor core and the surrounding peritumoral space. Our data provide a detailed dissection of GBM cell types, revealing an abundance of novel information about tumor formation and migration.