RT Journal Article SR Electronic T1 Single-Cell RNAseq analysis of infiltrating neoplastic cells at the migrating front of human glioblastoma JF bioRxiv FD Cold Spring Harbor Laboratory SP 165811 DO 10.1101/165811 A1 Spyros Darmanis A1 Steven A. Sloan A1 Derek Croote A1 Marco Mignardi A1 Sophia Chernikova A1 Peyman Samghababi A1 Ye Zhang A1 Norma Neff A1 Mark Kowarsky A1 Christine Caneda A1 Gordon Li A1 Steven Chang A1 Ian David Connolly A1 Yingmei Li A1 Ben Barres A1 Melanie Hayden Gephart A1 Stephen R. Quake YR 2017 UL http://biorxiv.org/content/early/2017/07/19/165811.abstract AB Glioblastoma is the most common primary brain cancer in adults and is notoriously difficult to treat due to its diffuse nature. We performed single-cell RNAseq on 3589 cells in a cohort of four patients. We obtained cells from the tumor core as well as surrounding peripheral tissue. Our analysis revealed cellular variation in the tumor’s genome and transcriptome, We were able to identify infiltrating neoplastic cells in regions peripheral to the core lesions. Despite the existence of significant heterogeneity among neoplastic cells, we found that infiltrating GBM cells share a consistent gene signature between patients, suggesting a common mechanism of infiltration. Additionally, in investigating the immunological response to the tumors, we found transcriptionally distinct myeloid cell populations residing in the tumor core and the surrounding peritumoral space. Our data provide a detailed dissection of GBM cell types, revealing an abundance of novel information about tumor formation and migration.