RT Journal Article SR Electronic T1 Increased expression of schizophrenia-associated gene C4 leads to hypoconnectivity of prefrontal cortex and reduced social interaction JF bioRxiv FD Cold Spring Harbor Laboratory SP 598342 DO 10.1101/598342 A1 Ashley L. Comer A1 Tushare Jinadasa A1 Lisa N. Kretsge A1 Thanh P.H. Nguyen A1 Jungjoon Lee A1 Elena R. Newmark A1 Frances S. Hausmann A1 SaraAnn Rosenthal A1 Kevin Liu Kot A1 William W. Yen A1 Alberto Cruz-Martín YR 2019 UL http://biorxiv.org/content/early/2019/04/04/598342.abstract AB Schizophrenia is a severe mental disorder with an unclear pathophysiology. Increased expression of the immune gene C4 has been linked to a greater risk of developing schizophrenia; however, it is unknown whether C4 plays a causative role in this brain disorder. Using confocal imaging and whole-cell electrophysiology, we demonstrate that overexpression of C4 in mouse prefrontal cortex neurons leads to perturbations in dendritic spine development and hypoconnectivity, which mirror neuropathologies found in schizophrenia. We find evidence that microglia-neuron interactions and microglia-mediated synaptic engulfment are enhanced with increased expression of C4. We also show that C4-dependent circuit dysfunction in the frontal cortex leads to decreased social interactions in juvenile mice. These results demonstrate that increased expression of the schizophrenia-associated gene C4 causes aberrant circuit wiring in the developing prefrontal cortex and leads to deficits in early social behavior, suggesting that altered C4 expression contributes directly to schizophrenia pathogenesis.