%0 Journal Article %A Hannes Ruge %A Theo A. J. Schäfer %A Katharina Zwosta %A Holger Mohr %A Uta Wolfensteller %T Initial neural representation of individual rules after first-time instruction %D 2019 %R 10.1101/598276 %J bioRxiv %P 598276 %X By following explicit instructions humans can instantaneously get the hang of tasks they have never performed before. Here, we used a specially calibrated multivariate analysis technique to uncover the elusive representational states following newly instructed arbitrary behavioural rules such as ‘for coffee, press red button’, while transitioning from ‘knowing what to do’ to ‘actually doing it’. Subtle variation in distributed neural activity patterns reflected rule-specific representations within the ventrolateral prefrontal cortex (VLPFC), confined to instructed stimulus-response learning in contrast to incidental learning involving the same stimuli and responses. VLPFC representations were established right after first-time instruction and remained stable across early implementation trials. More and more fluent application of novel rule representations was channelled through increasing cooperation between VLPFC and anterior striatum. These findings inform representational theories on how the prefrontal cortex supports behavioural flexibility by enabling ad-hoc coding of novel task rules without recourse to familiar sub-routines %U https://www.biorxiv.org/content/biorxiv/early/2019/04/05/598276.full.pdf