PT  - JOURNAL ARTICLE
AU  - Fanning Zeng
AU  - Valerie Beck
AU  - Sven Schuierer
AU  - Isabelle Garnier
AU  - Carole Manneville
AU  - Claudia Agarinis
AU  - Lapo Morelli
AU  - Lisa Quinn
AU  - Judith Knehr
AU  - Guglielmo Roma
AU  - Frederic Bassilana
AU  - Mark Nash
TI  - A simple and efficient CRISPR technique for protein tagging
AID  - 10.1101/597971
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 597971
4099  - http://biorxiv.org/content/early/2019/04/05/597971.short
4100  - http://biorxiv.org/content/early/2019/04/05/597971.full
AB  - Genetic knock-in using homology directed repair is an inefficient process, requiring selection of few modified cells and hindering its application to primary cells. Here we describe Homology independent gene Tagging (HiTag), a method to tag a protein of interest by CRISPR in up to 66% transfected cells with one single electroporation. The technique has proven effective in various cell types, can be used to knock in a fluorescent protein for live cell imaging, to modify the cellular location of a target protein and to monitor levels of a protein of interest by a luciferase assay in primary cells.