TY - JOUR T1 - A simple and efficient CRISPR technique for protein tagging JF - bioRxiv DO - 10.1101/597971 SP - 597971 AU - Fanning Zeng AU - Valerie Beck AU - Sven Schuierer AU - Isabelle Garnier AU - Carole Manneville AU - Claudia Agarinis AU - Lapo Morelli AU - Lisa Quinn AU - Judith Knehr AU - Guglielmo Roma AU - Frederic Bassilana AU - Mark Nash Y1 - 2019/01/01 UR - http://biorxiv.org/content/early/2019/04/05/597971.abstract N2 - Genetic knock-in using homology directed repair is an inefficient process, requiring selection of few modified cells and hindering its application to primary cells. Here we describe Homology independent gene Tagging (HiTag), a method to tag a protein of interest by CRISPR in up to 66% transfected cells with one single electroporation. The technique has proven effective in various cell types, can be used to knock in a fluorescent protein for live cell imaging, to modify the cellular location of a target protein and to monitor levels of a protein of interest by a luciferase assay in primary cells. ER -