RT Journal Article
SR Electronic
T1 The TAF10-containing TFIID and SAGA transcriptional complexes are dispensable for early somitogenesis in the mouse embryo
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 071324
DO 10.1101/071324
A1 Paul Bardot
A1 Stéphane D. Vincent
A1 Marjorie Fournier
A1 Alexis Hubaud
A1 Mathilde Joint
A1 László Tora
A1 Olivier Pourquié
YR 2017
UL http://biorxiv.org/content/early/2017/07/24/071324.abstract
AB During development, tightly regulated gene expression programs control cell fate and patterning. A key regulatory step in eukaryotic transcription is the assembly of the pre-initiation complex (PIC) at promoters. The PIC assembly has mainly been studied in vitro, and little is known about its composition during development. In vitro data suggests that TFIID is the general transcription factor that nucleates PIC formation at promoters. Here we show that TAF10, a subunit of TFIID and of the transcriptional co-activator SAGA, is required for the assembly of these complexes in the mouse embryo. We performed Taf10 conditional deletions during mesoderm development and show that Taf10 loss in the presomitic mesoderm (PSM) does not prevent cyclic gene transcription or PSM segmental patterning, while lateral plate differentiation is profoundly altered. During this period, global mRNA levels are unchanged in the PSM, with only a minor subset of genes dysregulated. Together, our data demonstrate that the TAF10-containing canonical TFIID and SAGA complexes, are dispensable for early paraxial mesoderm development, arguing against the generic role in transcription proposed for these fully assembled holo complexes.