RT Journal Article
SR Electronic
T1 A distinct cardiopharyngeal mesoderm genetic hierarchy establishes antero-posterior patterning of esophagus striated muscle
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 600841
DO 10.1101/600841
A1 Glenda Comai
A1 Églantine Heude
A1 Sebastien Mella
A1 Sylvain Paisant
A1 Francesca Pala
A1 Mirialys Gallardo
A1 Gabrielle Kardon
A1 Swetha Gopalakrishnan
A1 Shahragim Tajbakhsh
YR 2019
UL http://biorxiv.org/content/early/2019/04/05/600841.abstract
AB In most vertebrates, the upper digestive tract is composed of muscularised jaws linked to the esophagus that permit food uptake and swallowing. Masticatory and esophagus striated muscles (ESM) share a common cardiopharyngeal mesoderm (CPM) origin, however ESM are unusual among striated muscles as they are established in the absence of a primary skeletal muscle scaffold. Using mouse chimeras, we show that the transcription factors Tbx1 and Isl1 are required cell-autonomously for myogenic specification of ESM progenitors. Further, genetic loss-of-function and pharmacological studies point to Met/HGF signalling for antero-posterior migration of esophagus muscle progenitors, where HGF ligand is expressed in adjacent smooth muscle cells. These observations highlight the functional relevance of a smooth and striated muscle progenitor dialogue for ESM patterning. Our findings establish a Tbx1-Isl1-Met genetic hierarchy that uniquely regulate esophagus myogenesis and identify distinct genetic signatures that can be used as a framework to interpret pathologies arising within CPM derivatives.