TY - JOUR T1 - zUMIs: A fast and flexible pipeline to process RNA sequencing data with UMIs JF - bioRxiv DO - 10.1101/153940 SP - 153940 AU - Swati Parekh AU - Christoph Ziegenhain AU - Beate Vieth AU - Wolfgang Enard AU - Ines Hellmann Y1 - 2017/01/01 UR - http://biorxiv.org/content/early/2017/07/26/153940.abstract N2 - RNA sequencing is increasingly performed with less starting material and at a higher sample throughput, e.g. to analyse single-cell transcriptomes. In this context, unique molecular identifiers (UMIs) are used to reduce amplification noise and sample-specific barcodes are used to track libraries. Here, we present a fast and flexible pipeline to process data from such RNA-seq protocols.Availability: https://github.com/sdparekh/zUMIs ER -