RT Journal Article
SR Electronic
T1 Evaluation of Topramezone on Zebrafish Retinoid Signaling
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 167296
DO 10.1101/167296
A1 Haixing Liu
A1 Pengxing Xu
A1 Yu Fan
A1 Weizhi Zhang
YR 2017
UL http://biorxiv.org/content/early/2017/07/26/167296.abstract
AB Topramezone is a highly selective herbicide developed for broadleaf and grass weeds control in corn. In this study, the effects of topramezone on zebrafish, especially in retinoid signaling were investigated. Zebrafish embryos were treated with topramezone from 4 hours post-fertilization (hpf) to 144 hpf. Exposed to topramezone significantly reduced the retinal and retinoic levels compared to controls. The transcriptional expression levels of retinol dehydrogenase (rdh1), retinoic acid receptor subunit (raraa), retinal dehydrogenase (raldh2), retinol binding protein (rbp1a), and cellular retinoic acid binding protein (crabp1a and crabp2a) were significantly decreased. Our results suggested that topramezone significantly impaired zebrafish retinoid signaling during a short time exposure. However, treatment with topramezone significantly increased the mRNA expression levels of zfblue, zfrho, zfgr1, zfuv, and zfred. Our data demonstrated that topramezone treatment could interrupt retinoid signaling and further affect zebrafish eye development.