PT  - JOURNAL ARTICLE
AU  - Genevieve E Martin
AU  - Matthew Pace
AU  - John P Thornhill
AU  - Chansavath Phetsouphanh
AU  - Jodi Meyerowitz
AU  - Morgane Gossez
AU  - Emily Hopkins
AU  - Helen Brown
AU  - Nicola Robinson
AU  - Natalia Olejniczak
AU  - Gita Ramjee
AU  - Pontiano Kaleebu
AU  - Kholoud Porter
AU  - Christian Willberg
AU  - Paul Klenerman
AU  - Nneka Nwokolo
AU  - Julie Fox
AU  - Sarah Fidler
AU  - John Frater
TI  - Enrichment of the HIV reservoir in CD32+ CD4 T cells occurs early in blood and tissue
AID  - 10.1101/169342
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 169342
4099  - http://biorxiv.org/content/early/2017/07/27/169342.short
4100  - http://biorxiv.org/content/early/2017/07/27/169342.full
AB  - The Fc receptor CD32 has been proposed as a marker for CD4 T cells latently infected with HIV. We demonstrate that enrichment for HIV DNA in CD32+ CD4 T cells can be found early in infection in both tissue and blood. However, we find no evidence for a correlation between CD32 expression on CD4 T cells and either HIV DNA levels or time to rebound viraemia following treatment interruption. CD32+ CD4 T cells have a more differentiated memory phenotype, and high levels of expression of immune checkpoint receptors PD-1, Tim-3 and TIGIT as well as the activation marker, HLA DR. There was no difference in the phenotype or frequency of CD32 expressing cells prior to or after the initiation of antiretroviral therapy, or compared with healthy controls, suggesting that preferential infection or survival, rather than up-regulation, may be responsible for the observed enrichment of proviral HIV DNA in CD32+ CD4 T cells.