RT Journal Article
SR Electronic
T1 Enrichment of the HIV reservoir in CD32+ CD4 T cells occurs early in blood and tissue
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 169342
DO 10.1101/169342
A1 Genevieve E Martin
A1 Matthew Pace
A1 John P Thornhill
A1 Chansavath Phetsouphanh
A1 Jodi Meyerowitz
A1 Morgane Gossez
A1 Emily Hopkins
A1 Helen Brown
A1 Nicola Robinson
A1 Natalia Olejniczak
A1 Gita Ramjee
A1 Pontiano Kaleebu
A1 Kholoud Porter
A1 Christian Willberg
A1 Paul Klenerman
A1 Nneka Nwokolo
A1 Julie Fox
A1 Sarah Fidler
A1 John Frater
YR 2017
UL http://biorxiv.org/content/early/2017/07/27/169342.abstract
AB The Fc receptor CD32 has been proposed as a marker for CD4 T cells latently infected with HIV. We demonstrate that enrichment for HIV DNA in CD32+ CD4 T cells can be found early in infection in both tissue and blood. However, we find no evidence for a correlation between CD32 expression on CD4 T cells and either HIV DNA levels or time to rebound viraemia following treatment interruption. CD32+ CD4 T cells have a more differentiated memory phenotype, and high levels of expression of immune checkpoint receptors PD-1, Tim-3 and TIGIT as well as the activation marker, HLA DR. There was no difference in the phenotype or frequency of CD32 expressing cells prior to or after the initiation of antiretroviral therapy, or compared with healthy controls, suggesting that preferential infection or survival, rather than up-regulation, may be responsible for the observed enrichment of proviral HIV DNA in CD32+ CD4 T cells.