RT Journal Article
SR Electronic
T1 Deciphering the protein dynamics and molecular determinants of iPSC-derived neurons
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 599415
DO 10.1101/599415
A1 Suzy Varderidou-Minasian
A1 Philipp Schätzle
A1 Casper. C. Hoogenraad
A1 R. Jeroen Pasterkamp
A1 Maarten Altelaar
YR 2019
UL http://biorxiv.org/content/early/2019/04/06/599415.abstract
AB Neuronal development is a multistep process with different regulatory programs that shapes neurons to form dendrites, axons and synapses. To date, knowledge on neuronal development is largely based on murine data and largely restricted to the genomic and transcriptomic level. Advances in stem cell differentiation now enable the study of human neuronal development, and here we provide a mass spectrometry-based quantitative proteomic signature, at high temporal resolution, of human stem cell-derived neurons. To reveal proteomic changes during neuronal development we make use of two differentiation approaches, either by expression of neurogenin-2 (Ngn2) leading to glutamatergic induced neurons (iN) or via small molecule manipulations, leading to patterned motor neurons. Our analysis revealed key proteins that show significant expression changes (FDR <0.001) during neuronal differentiation. We overlay our proteomics data with available transcriptomic data during neuronal differentiation and show distinct, datatype-specific, signatures. Overall, we provide a rich resource of information on proteins associated with human neuronal development, and moreover, highlight several signaling pathways involved, such as Wnt and Notch.