TY - JOUR T1 - Protein phosphatase V ensures timely cell cycle remodeling during the mid-blastula transition in Drosophila JF - bioRxiv DO - 10.1101/172379 SP - 172379 AU - Boyang Liu AU - Hung-wei Sung AU - Ingo Gregor AU - H.-Arno Müller AU - Jörg Großhans Y1 - 2017/01/01 UR - http://biorxiv.org/content/early/2017/08/04/172379.abstract N2 - Cell cycle remodeling from fast nuclear cycles to a generic cell cycle mode is a major feature of the mid-blastula transition (MBT) in Drosophila. Remodeling occurs when Twine/Cdc25 falls below a critical threshold. Timing is based on Twine destabilization induced by zygotic transcription. It is conceivable that appropriate starting levels are also important for timely reaching the threshold. Mechanisms for controlling Twine levels at the onset of MBT are unknown. Here we identify a function of the protein phosphatase V in this mechanism. Twine was increased in PpV mutants, whereas the decay rate was comparable to wildtype. PpV mutants frequently underwent an extra nuclear division. We detected PpV-dependent phosphosites in Twine. Phosphosite mutants contain higher Twine levels and frequently underwent an extra nuclear division, comparable to PpV mutants. Our data support a model that the cell cycle remodeling is controlled by induced destabilization and PpV-d ependent control of Twine levels. ER -