PT  - JOURNAL ARTICLE
AU  - Israel S. Fernández
AU  - V. Ramakrishnan
TI  - GTP Hydrolysis by eIF5B in the Last Step of Translation Initiation Is Activated by a Rotation of the Small Ribosomal Subunit
AID  - 10.1101/172825
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 172825
4099  - http://biorxiv.org/content/early/2017/08/04/172825.short
4100  - http://biorxiv.org/content/early/2017/08/04/172825.full
AB  - Placement of an initiator aminoacyl-tRNA [(f)Met-tRNAi(f)Met] base paired with the AUG initiation codon of a messenger RNA (mRNA) is the first step of translation. The eukaryotic translation factor eIF5B or its bacerial homologue IF2 facilitate the correct positioning of initiator tRNA in the P site of the ribosome. We report the electron cryomicroscopy (cryoEM) structure of a stabilized intermediate state of a yeast 80S/tRNAiMet/eIF5B complex at 3.6 Å resolution. The structure shows how a universally conserved tyrosine couples the rotational state of the small ribosomal subunit with GTP hydrolysis.