RT Journal Article
SR Electronic
T1 Asymmetry of an intracellular scaffold at vertebrate electrical synapses
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 173955
DO 10.1101/173955
A1 Audrey J Marsh
A1 Jennifer Carlisle Michel
A1 Anisha P Adke
A1 Emily L Heckman
A1 Adam C Miller
YR 2017
UL http://biorxiv.org/content/early/2017/08/09/173955.abstract
AB Neuronal synaptic connections are electrical or chemical and together are essential to dynamically defining neural circuit function. While chemical synapses are well known for their biochemical complexity, electrical synapses are often viewed as comprised solely of neuronal gap junction channels that allow direct ionic and metabolic communication. However, associated with the gap junction channels are structures observed by electron microscopy called the Electrical Synapse Density (ESD). The ESD has been suggested to be critical for the formation and function of the electrical synapse, yet the biochemical makeup of these structures is poorly understood. Here we find that electrical synapse formation in vivo requires an intracellular scaffold called Tight Junction Protein 1b (Tjp1b). Tjp1b is localized to electrical synapses where it is required for the stabilization of the gap junction channels and for electrical synapse function. Strikingly, we find that Tjp1b protein localizes and functions asymmetrically, exclusively on the postsynaptic side of the synapse. Our findings support a novel model in which there is molecular asymmetry at the level of the intracellular scaffold that is required for building the electrical synapse. ESD molecular asymmetries may be a fundamental motif of all nervous systems and could support functional asymmetry at the electrical synapse.