PT  - JOURNAL ARTICLE
AU  - Yiming Li
AU  - Markus Mund
AU  - Philipp Hoess
AU  - Ulf Matti
AU  - Bianca Nijmeijer
AU  - Vilma Jimenez Sabinina
AU  - Jan Ellenberg
AU  - Ingmar Schoen
AU  - Jonas Ries
TI  - Fast, robust and precise 3D localization for arbitrary point spread functions
AID  - 10.1101/172643
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 172643
4099  - http://biorxiv.org/content/early/2017/08/10/172643.short
4100  - http://biorxiv.org/content/early/2017/08/10/172643.full
AB  - We present a fitter for 3D single-molecule localization of arbitrary, experimental point spread functions (PSFs) that reaches minimum uncertainty for EMCCD and sCMOS cameras, and achieves more than 105 fits/s. We provide tools to robustly model experimental PSFs and correct for depth induced aberrations, which allowed us to achieve an unprecedented 3D resolution with engineered astigmatic PSFs, and acquire high quality 3D superresolution images even on standard microscopes without 3D optics.