@article {Gaysinskaya177535, author = {Valeriya Gaysinskaya and Brendan F. Miller and Godfried W. van der Heijden and Kasper D. Hansen and Alex Bortvin}, title = {Transient Reduction of DNA Methylation at the Onset of Meiosis in Male Mice}, elocation-id = {177535}, year = {2017}, doi = {10.1101/177535}, publisher = {Cold Spring Harbor Laboratory}, abstract = {The quality of germ cells depends on successful chromatin organization in meiotic prophase I (MPI). To better understand the epigenetic context of MPI we studied the dynamics of DNA methylation in wild-type male mice. We discovered an extended period of genome-wide transient reduction of DNA methylation (TRDM) during early MPI. Our data show that TRDM arises by passive demethylation in the premeiotic S phase highlighting the abundance of hemimethylated DNA in MPI. Importantly, TRDM unmasks a deficit in retrotransposon LINE-1 DNA methylation contributing to its expression in early MPI. We propose that TRDM facilitates meiosis and gamete quality control.}, URL = {https://www.biorxiv.org/content/early/2017/08/17/177535}, eprint = {https://www.biorxiv.org/content/early/2017/08/17/177535.full.pdf}, journal = {bioRxiv} }