%0 Journal Article %A Valeriya Gaysinskaya %A Brendan F. Miller %A Godfried W. van der Heijden %A Kasper D. Hansen %A Alex Bortvin %T Transient Reduction of DNA Methylation at the Onset of Meiosis in Male Mice %D 2017 %R 10.1101/177535 %J bioRxiv %P 177535 %X The quality of germ cells depends on successful chromatin organization in meiotic prophase I (MPI). To better understand the epigenetic context of MPI we studied the dynamics of DNA methylation in wild-type male mice. We discovered an extended period of genome-wide transient reduction of DNA methylation (TRDM) during early MPI. Our data show that TRDM arises by passive demethylation in the premeiotic S phase highlighting the abundance of hemimethylated DNA in MPI. Importantly, TRDM unmasks a deficit in retrotransposon LINE-1 DNA methylation contributing to its expression in early MPI. We propose that TRDM facilitates meiosis and gamete quality control. %U https://www.biorxiv.org/content/biorxiv/early/2017/08/17/177535.full.pdf