@article {van der Wijst177568, author = {M.G.P. van der Wijst and H. Brugge and D.H. de Vries and L.H. Franke}, title = {Single-cell RNA sequencing reveals cell-type specific cis-eQTLs in peripheral blood mononuclear cells}, elocation-id = {177568}, year = {2017}, doi = {10.1101/177568}, publisher = {Cold Spring Harbor Laboratory}, abstract = {Most disease-associated genetic risk factors are regulatory. Here, we generated single-cell RNA-seq data of \~{}25,000 peripheral blood mononuclear cells from 45 donors to identify how genetic variants affect gene expression. We validated this approach by replicating previously published whole blood RNA-seq cis-expression quantitative trait loci effects (cis-eQTLs), but also identified new cell type-specific cis-eQTLs. These eQTLs give additional insight into the downstream consequences of genetic risk factors for immune-mediated diseases.}, URL = {https://www.biorxiv.org/content/early/2017/08/17/177568}, eprint = {https://www.biorxiv.org/content/early/2017/08/17/177568.full.pdf}, journal = {bioRxiv} }