PT  - JOURNAL ARTICLE
AU  - M.G.P. van der Wijst
AU  - H. Brugge
AU  - D.H. de Vries
AU  - L.H. Franke
TI  - Single-cell RNA sequencing reveals cell-type specific <em>cis</em>-eQTLs in peripheral blood mononuclear cells
AID  - 10.1101/177568
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 177568
4099  - http://biorxiv.org/content/early/2017/08/17/177568.short
4100  - http://biorxiv.org/content/early/2017/08/17/177568.full
AB  - Most disease-associated genetic risk factors are regulatory. Here, we generated single-cell RNA-seq data of ∼25,000 peripheral blood mononuclear cells from 45 donors to identify how genetic variants affect gene expression. We validated this approach by replicating previously published whole blood RNA-seq cis-expression quantitative trait loci effects (cis-eQTLs), but also identified new cell type-specific cis-eQTLs. These eQTLs give additional insight into the downstream consequences of genetic risk factors for immune-mediated diseases.