RT Journal Article
SR Electronic
T1 Single-cell RNA sequencing reveals cell-type specific <em>cis</em>-eQTLs in peripheral blood mononuclear cells
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 177568
DO 10.1101/177568
A1 M.G.P. van der Wijst
A1 H. Brugge
A1 D.H. de Vries
A1 L.H. Franke
YR 2017
UL http://biorxiv.org/content/early/2017/08/17/177568.abstract
AB Most disease-associated genetic risk factors are regulatory. Here, we generated single-cell RNA-seq data of ∼25,000 peripheral blood mononuclear cells from 45 donors to identify how genetic variants affect gene expression. We validated this approach by replicating previously published whole blood RNA-seq cis-expression quantitative trait loci effects (cis-eQTLs), but also identified new cell type-specific cis-eQTLs. These eQTLs give additional insight into the downstream consequences of genetic risk factors for immune-mediated diseases.