RT Journal Article
SR Electronic
T1 Transient Reduction of DNA Methylation at the Onset of Meiosis in Male Mice
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 177535
DO 10.1101/177535
A1 Gaysinskaya, Valeriya
A1 Miller, Brendan F.
A1 van der Heijden, Godfried W.
A1 Hansen, Kasper D.
A1 Bortvin, Alex
YR 2017
UL http://biorxiv.org/content/early/2017/08/17/177535.1.abstract
AB The quality of germ cells depends on successful chromatin organization in meiotic prophase I (MPI). To better understand the epigenetic context of MPI we studied the dynamics of DNA methylation in wild-type male mice. We discovered an extended period of genome-wide transient reduction of DNA methylation (TRDM) during early MPI. Our data show that TRDM arises by passive demethylation in the premeiotic S phase highlighting the abundance of hemimethylated DNA in MPI. Importantly, TRDM unmasks a deficit in retrotransposon LINE-1 DNA methylation contributing to its expression in early MPI. We propose that TRDM facilitates meiosis and gamete quality control.