PT  - JOURNAL ARTICLE
AU  - Delphine Dardalhon-Cuménal
AU  - Jérôme Deraze
AU  - Camille A Dupont
AU  - Valérie Ribeiro
AU  - Anne Coléno-Costes
AU  - Juliette Pouch
AU  - Stéphane Le Crom
AU  - Hélène Thomassin
AU  - Vincent Debat
AU  - Neel B Randsholt
AU  - Frédérique Peronnet
TI  - Cyclin G and the Polycomb Repressive Complexes PRC1 and PR-DUB cooperate for developmental stability
AID  - 10.1101/144048
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 144048
4099  - http://biorxiv.org/content/early/2017/08/20/144048.short
4100  - http://biorxiv.org/content/early/2017/08/20/144048.full
AB  - In Drosophila, ubiquitous expression of a short Cyclin G isoform generates extreme developmental noise estimated by fluctuating asymmetry (FA), providing a model to tackle developmental stability. This transcriptional cyclin interacts with chromatin regulators of the Enhancer of Trithorax and Polycomb (ETP) and Polycomb families. We investigate here the importance of these interactions in developmental stability. Deregulation of Cyclin G highlights an organ intrinsic control of developmental noise, linked to the ETP-interacting domain, and enhanced by mutations in genes encoding members of the Polycomb Repressive complexes PRC1 and PR-DUB. Deep-sequencing of wing imaginal discs deregulating CycG reveals that high developmental noise correlates with up-regulation of genes involved in translation and down-regulation of genes involved in energy production. Most Cyclin G direct transcriptional targets are also direct targets of PRC1, ASX and RNAPolII in the developing wing. Altogether, our results suggest that Cyclin G, PRC1 and PR-DUB cooperate for developmental stability.