RT Journal Article
SR Electronic
T1 Targeting individual cells by barcode in pooled sequence libraries
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 178681
DO 10.1101/178681
A1 Navpreet Ranu
A1 Alexandra-Chloé Villani
A1 Nir Hacohen
A1 Paul C. Blainey
YR 2017
UL http://biorxiv.org/content/early/2017/08/20/178681.abstract
AB There is rising interest in applying single-cell transcriptome analysis and other single-cell sequencing methods to resolve differences between cells. Pooled processing of thousands of single cells is now routinely practiced by introducing cell-specific DNA barcodes early in cell processing protocols1-5. However, researchers must sequence a large number of cells to sample rare subpopulations6-8, even when fluorescence-activated cell sorting (FACS) is used to pre-enrich rare cell populations. Here, a new molecular enrichment method is used in conjunction with FACS enrichment to enable efficient sampling of rare dendritic cell (DC) populations, including the recently identified AXL+SIGLEC6+ (AS DCs) subset7, within a 10X Genomics single-cell RNA-Seq library. DC populations collectively represent 1-2% of total peripheral blood mononuclear cells (PBMC), with AS DC representing only 1-3% of human blood DCs and 0.01-0.06% of total PBMCs.