RT Journal Article
SR Electronic
T1 Ultra-fast super-resolution imaging of biomolecular mobility in tissues
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 179747
DO 10.1101/179747
A1 Helen Miller
A1 Jason Cosgrove
A1 Adam J. M. Wollman
A1 Peter O’ J. Toole
A1 Mark C. Coles
A1 Mark C. Leake
YR 2017
UL http://biorxiv.org/content/early/2017/08/23/179747.abstract
AB Super-resolution techniques have addressed many biological questions, yet molecular quantification at rapid timescales in live tissues remains challenging. We developed a light microscopy system capable of sub-millisecond sampling to characterize molecular diffusion in heterogeneous aqueous environments comparable to interstitial regions between cells in tissues. We demonstrate our technique with super-resolution tracking of fluorescently labelled chemokine molecules in a collagen matrix and ex vivo lymph node tissue sections, outperforming competing methods.