RT Journal Article
SR Electronic
T1 A plant receptor-like kinase promotes cell-to-cell spread of RNAi and is targeted by a virus
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 180380
DO 10.1101/180380
A1 Tabata Rosas-Diaz
A1 Dan Zhang
A1 Pengfei Fan
A1 Liping Wang
A1 Xue Ding
A1 Yuli Jiang
A1 Tamara Jimenez-Gongora
A1 Laura Medina-Puche
A1 Xinyan Zhao
A1 Zhengyan Feng
A1 Guiping Zhang
A1 Xiaokun Liu
A1 Eduardo R Bejarano
A1 Li Tan
A1 Jian-Kang Zhu
A1 Weiman Xing
A1 Christine Faulkner
A1 Shingo Nagawa
A1 Rosa Lozano-Duran
YR 2017
UL http://biorxiv.org/content/early/2017/08/24/180380.abstract
AB RNA interference (RNAi) in plants can move from cell to cell, allowing for systemic spread of an anti-viral immune response. How this cell-to-cell spread of silencing is regulated is currently unknown. Here, we describe that the C4 protein from Tomato yellow leaf curl virus has the ability to inhibit the intercellular spread of RNAi. Using this viral protein as a probe, we have identified the receptor-like kinase (RLK) BARELY ANY MERISTEM 1 (BAM1) as a positive regulator of the cell-to-cell movement of RNAi, and determined that BAM1 and its closest homologue, BAM2, play a redundant role in this process. C4 interacts with the intracellular domain of BAM1 and BAM2 at the plasma membrane and plasmodesmata, the cytoplasmic connections between plant cells, interfering with the function of these RLKs in the cell-to-cell spread of RNAi. Our results identify BAM1 as an element required for the cell-to-cell spread of RNAi and highlight that signalling components have been co-opted to play multiple functions in plants.