TY - JOUR T1 - Changes in free amino acid concentrations and associated gene expression profiles in the abdominal muscle of kuruma shrimp <em>Marsupenaeus japonicus</em> reared at different salinity JF - bioRxiv DO - 10.1101/180638 SP - 180638 AU - Hiroki Koyama AU - Nanami Mizusawa AU - Masataka Hoashi AU - Enkong Tan AU - Ko Yasumoto AU - Mitsuru Jimbo AU - Daisuke Ikeda AU - Takehiko Yokoyama AU - Shuichi Asakawa AU - Sanit Piyapattanakorn AU - Shugo Watabe Y1 - 2017/01/01 UR - http://biorxiv.org/content/early/2017/08/25/180638.abstract N2 - Shrimps inhabiting around the coastal area can survive in a wide range of salinity. However, the molecular mechanisms involved in their adaptation to different environmental salinity have remained largely unknown. In the present study, we reared kuruma shrimp Marsupenaeus japonicus at 1.7 %, 3.4 % and 4.0 % salinity. After rearing for 6, 12, 24 and 72 h, we determined free amino acid concentrations in their abdominal muscle, and performed RNA-seq analysis on this muscle. The concentrations of free amino acids were clearly altered depending on salinity after rearing for 24 h. Glutamine and alanine concentrations were markedly increased following the increase of salinity. In association with such changes, many genes related to amino acid metabolism changed their expression levels. Notably, the increased glutamine content at high salinity appeared to be relevant to the increase of the expression level of the gene encoding glutamate-ammonia ligase which functions in the glutamine metabolism. Furthermore, the alanine content increased at high salinity was likely to be associated with the decrease in the expression levels of the alanine-glyoxylate transaminase gene. Thus, the changes in the concentration of free amino acids for osmoregulation in kuruma shrimp are considered to be regulated by the changes in the expression levels of genes related to amino acid metabolism.Summary statesment Kuruma shrimp Marsupenaeus japonicus changes free amino acid contents and associated gene expression levels in their muscle to adjust effectively to different salinity. ER -