PT  - JOURNAL ARTICLE
AU  - Ryoji Amamoto
AU  - Emanuela Zuccaro
AU  - Nathan C. Curry
AU  - Sonia Khurana
AU  - Hsu-Hsin Chen
AU  - Constance L. Cepko
AU  - Paola Arlotta
TI  - FIN-Seq: Transcriptional profiling of specific cell types in frozen archived tissue from the human central nervous system
AID  - 10.1101/602847
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 602847
4099  - http://biorxiv.org/content/early/2019/04/08/602847.short
4100  - http://biorxiv.org/content/early/2019/04/08/602847.full
AB  - Thousands of frozen, archived tissues from postmortem human central nervous system (CNS) are currently available in brain banks. As single cell and single nucleus technologies are beginning to elucidate the cellular diversity present within the human CNS, it is becoming clear that transcriptional analysis of the human CNS requires cell type specificity. Single cell and single nucleus RNA profiling provide one avenue to decipher this heterogeneity. An alternative, complementary approach is to profile isolated, pre-defined cell types and use methods that can be applied to many archived human tissue samples. Here, we developed FIN-Seq (Frozen Immunolabeled Nuclei Sequencing), a method that accomplishes these goals. FIN-Seq uses immunohisto-chemical isolation of nuclei of specific cell types from frozen human tissue, followed by RNA-Sequencing. We applied this method to frozen postmortem samples of human cerebral cortex and retina and were able to identify transcripts, including low abundance transcripts, in specific cell types.