RT Journal Article
SR Electronic
T1 An improved ATAC-seq protocol reduces background and enables interrogation of frozen tissues
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 181206
DO 10.1101/181206
A1 M. Ryan Corces
A1 Alexandro E. Trevino
A1 Emily G. Hamilton
A1 Peyton G. Greenside
A1 Nicholas A. Sinnott-Armstrong
A1 Sam Vesuna
A1 Ansuman T. Satpathy
A1 Adam J. Rubin
A1 Kathleen S. Montine
A1 Beijing Wu
A1 Arwa Kathiria
A1 Seung Woo Cho
A1 Maxwell R. Mumbach
A1 Ava C. Carter
A1 Maya Kasowski
A1 Lisa A. Orloff
A1 Viviana I. Risca
A1 Anshul Kundaje
A1 Paul A. Khavari
A1 Thomas J. Montine
A1 William J. Greenleaf
A1 Howard Y. Chang
YR 2017
UL http://biorxiv.org/content/early/2017/08/26/181206.abstract
AB We present Omni-ATAC, an improved ATAC-seq protocol for chromatin accessibility profiling that works across multiple applications with substantial improvement of signal-to-background ratio and information content. The Omni-ATAC protocol enables chromatin accessibility profiling from archival frozen tissue samples and 50 μm sections, revealing the activities of disease-associated DNA elements in distinct human brain structures. The Omni-ATAC protocol enables the interrogation of personal regulomes in tissue context and translational studies.