PT  - JOURNAL ARTICLE
AU  - Min Guo
AU  - Panagiotis Chandris
AU  - John Paul Giannini
AU  - Adam J. Trexler
AU  - Robert Fischer
AU  - Jiji Chen
AU  - Harshad D. Vishwasrao
AU  - Ivan Rey-Suarez
AU  - Yicong Wu
AU  - Clare M. Waterman
AU  - George H. Patterson
AU  - Arpita Upadhyaya
AU  - Justin Taraska
AU  - Hari Shroff
TI  - Single-shot super-resolution total internal reflection fluorescence microscopy
AID  - 10.1101/182121
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 182121
4099  - http://biorxiv.org/content/early/2017/08/29/182121.short
4100  - http://biorxiv.org/content/early/2017/08/29/182121.full
AB  - We demonstrate a simple method for combining instant structured illumination microscopy (SIM) with total internal reflection fluorescence microscopy (TIRF), doubling the spatial resolution of TIRF (down to 115 +/-13 nm) and enabling imaging frame rates up to 100 Hz over hundreds of time points. We apply instant TIRF-SIM to multiple live samples, achieving rapid, high contrast super-resolution imaging in close proximity to the coverslip surface.