RT Journal Article
SR Electronic
T1 Light Sheet Theta Microscopy for High-resolution Quantitative Imaging of Large Biological Systems
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 119289
DO 10.1101/119289
A1 Bianca Migliori
A1 Malika S. Datta
A1 Christophe Dupre
A1 Mehmet C. Apak
A1 Shoh Asano
A1 Ruixuan Gao
A1 Edward S. Boyden
A1 Ola Hermanson
A1 Rafael Yuste
A1 Raju Tomer
YR 2017
UL http://biorxiv.org/content/early/2017/08/30/119289.abstract
AB Advances in tissue clearing and molecular labelling methods are enabling unprecedented optical access to large intact biological systems. These advances fuel the need for high-speed microscopy approaches to image large samples quantitatively and at high resolution. While Light Sheet Microscopy (LSM), with its high planar imaging speed and low photo-bleaching, can be effective, scaling up to larger imaging volumes has been hindered by the use of orthogonal light-sheet illumination. To address this fundamental limitation, we have developed Light Sheet Theta Microscopy (LSTM), which uniformly illuminates samples from same side as the detection objective, thereby eliminating limits on lateral dimensions without sacrificing imaging resolution, depth and speed. We present detailed characterization of LSTM, and show that this approach achieves rapid high-resolution imaging of large intact samples with superior uniform high-resolution than LSM. LSTM is a significant step in high-resolution quantitative mapping of structure and function of large intact biological systems.